Zearalenone is an oestrogenic mycotoxin produced by the fungus Fusarium graminearum. Zearalenone induces feminisation in animals at concentrations in feed of about 1 ppm. Higher concentrations (50-100 ppm) can interfere with conception, ovulation, implantation, fetal development, and the viability of newborn animals.
Using monoclonal antibody-based affinity chromatography ZearalaTest can be used for the detection of zearalenone using immunoaffinity columns and a fluorometer or HPLC.
ZearalaTest is safe and simple. It can be performed in less than 15 minutes and requires no special skills. Results may be recorded using a digital fluorometer and ZearalaTest is also ideal as the cleanup step for any HPLC or TLC analysis.
- Convenient – For use with fluorometer or HPLC
- Durable – Long shelf life; requires no refrigeration
- Versatile – For use with a variety of samples
- Quick – Less than 15 minutes to isolate toxin (excluding preparation and extraction)
- Wide Range – Detects levels as low as 0.1 ppm and as high as 5 ppm
- Safe – Requires less toxic materials than conventional methods
Using monoclonal antibody-based affinity chromatography, ZearalaTest™WB is a wide bore HPLC-only test for the detection of zearalenone using wide bore immunoaffinity columns.
With a total volume of 3mL, compared to the total of 1 mL in VICAM’s standard columns, ZearalaTest WB allows for the faster flow rate preferred by many laboratories and is the ideal cleanup step for any HPLC.