The Fumonisins (B1, B2, and B3) are a group of mycotoxins produced by Fusarium moniliforme. Fumonisins have been found worldwide as a contaminant of maize (corn) and have been shown to cause liver cancer in experimental rats, pulmonary oedema in pigs and leukoencephalomalacia in horses.
The Helica Fumonisin ELISA is a competitive ELISA intended for the quantitative detection of fumonisins in maize.
- A fumonisin-specific antibody optimized to cross react with the three fumonisin subtypes is coated to a polystyrene microwell.
- Toxins are extracted from a ground sample with 90% methanol.
- The extracted sample and HRP-conjugated fumonisin are mixed and added to the antibody-coated microwell.
- Fumonisin from the extracted sample and HRP-conjugated fumonisin compete to bind with the antibody coated to the microwell.
- Microwell contents are decanted and non-specific reactants are removed by washing.
- An enzyme substrate (TMB) is added and a blue colour develops.
- The intensity of the colour is directly proportional to the amount of bound conjugate and inversely proportional to the concentration of fumonisin in the sample or standard.