Mycotoxins|Lab Test Kits|Total Aflatoxin ELISA

Total Aflatoxin ELISA

Helica MycoMonitor Total Aflatoxin Assay is a competitive ELISA intended for the quantitative detection of total aflatoxins (B1, B2, G1 and G2) in grains, nuts, cottonseeds, cereals and other commodities including animal feeds.

Test Procedure

  • The polystyrene microwells provided are coated with aflatoxin-specific antibody that cross reacts with all four subtypes of aflatoxin .
  • Toxins are extracted from a ground sample with 70% methanol.
  • The extracted sample and HRP-conjugated aflatoxin B1 are mixed and added to the antibody-coated microwell.
  • Aflatoxin from the extracted sample and HRP-conjugated aflatoxin B1 compete to bind with the antibody coated to the microwell.
  • Microwell contents are decanted and non-specific reactants are removed by washing.
  • An enzyme substrate (TMB) is added and a blue colour develops.
  • The intensity of the colour is directly proportional to the amount of bound conjugate and inversely proportional to the concentration of aflatoxin in the sample or standard.

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