Allergen ELISAs

Our Allergen-Check ELISA kits cover 14 legislated allergens, they are quantitative and detect food allergens in fresh, processed and heated food products, as well as from rinse waters and environmental swabs. They are all based on a 60-minute (20:20:20) sandwich assay format and contain all the reagents required for extraction and detection. The kits are easily automated for even greater workflow efficiency.

There are 18 allergen-specific kits in the range, which are available in 48-well (made to order) and 96-well (stock item) format. All the Allergen-Check™ ELISAs contain extraction solution concentrates in the kit. The prepared sample extracts can be tested in all kits except the casein, crustacea, fish and milk kits which have different extractions.  The ELISA procedure shares a common approach for all kits, with 20+20+20 minute incubations, allowing the potential for automation. The kits are all highly sensitive and accurate with the following Limits of Quantitation (LOQ) and Limit of Detection (LOD):

Allergen-Check ELISALOQ*LOD*48 well 96 well 
Milk (β-LG)0.2-7.0 (SMP**)<0.1 (SMP)R6037R6036
Almond0.5-10<0.25R6019R6018
Milk (Casein)0.8-16 (SMP)<0.2 (SMP)R6023R6022
Cashew2-50<0.2R6047R6046
Crustacea1.2-24 (dry wt.)<0.1 (dry wt.)R6027R6026
Egg0.4-8<0.2R6033R6032
Fish5-125<1.0R6009R6010
Hazelnut1-40<0.5R6015R6014
Lysozyme0.025-0.250.002R6079R6080
Lupin2-30<0.3R6031R6030
Milk (Total)1-27<0.1R6041R6040
Mustard2-50<1R6013R6012
Ovalbumin0.025-0.50.004R6077R6078
Peanut1-30<0.5R6021R6020
Pistachio1-25<0.2R6043R6042
Sesame2-20<0.2R6029R6028
Soya2-25<0.7R6025R6024
Walnut2-50<1.0R6017R6016

* In mg allergen/kg levels NOT protein levels, which are lower.

**SMP: Skimmed Milk Powder

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