ELISAs

Bio-Check (UK) has developed five ELISA-based methods for the detection of ultra-low levels of raw meats (cow, horse, pig, poultry and sheep) in uncooked food products. All are semi-quantitative and enable the important differentiation of cross-contamination (0.1 – 0.5% w/w) from adulteration (1.0 – 5.0% w/w).

The Raw Meat Species-Check ELISA kits have been formatted and refined for ease-of-use to aid in resolution of species content. They are designed to enable labs to analyse raw meat species content semi-quantitatively in order to meet or exceed relevant guidelines and legislation. A rigorous in-house validation has been conducted to support their performance. The Raw Species-Check™ ELISAs are also suitable for monitoring surface residues from cleaning validation and verification studies.

  • Specific for: serum albumin protein
  • Limit of Detection: below 0.1% for meat (all species)
  • Limits of semi-Quantitation: 0.1 – 0.5% w/w (without diltuion), 1.0 – 5.0% w/w (with dilution)
  • Tests per Kit: in duplicate – Up to 22 samples/48 wells and up to 46 samples/96 wells
  • Hands-on-time: 10 minutes (sample preparation/extraction), 60 minutes (immunoassay)
  • Kit Format: 48 and 96 microwell plates (strips of 8 wells)
  • Additional equipment: Micro-pipettes (50-100μL), wash bottle/plate reader
Kit Name (number of wells)REF.
Raw COW-Check™ ELISA (48)R6104
Raw COW-Check™ ELISA (96)R6105
Raw HORSE-Check™ ELISA (48)R6106
Raw HORSE-Check™ ELISA (96)R6107
Raw PIG-Check™ ELISA (48)R6083
Raw PIG-Check™ ELISA (96)R6084
Raw POULTRY-Check™ ELISA (48)R6108
Raw POULTRY-Check™ ELISA (96)R6109
Raw SHEEP-Check™ ELISA (48)R6110
Raw SHEEP-Check™ ELISA (96)R6111

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